Search results for " tobacco"

showing 10 items of 51 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Cytosolic calcium rises and related events in ergosterol-treated Nicotiana cells

2011

International audience; The typical fungal membrane component ergosterol was previously shown to trigger defence responses and protect plants against pathogens. Most of the elicitors mobilize the second messenger calcium, to trigger plant defences. We checked the involvement of calcium in response to ergosterol using Nicotiana plumbaginifolia and Nicotiana tabacum cv Xanthi cells expressing apoaequorin in the cytosol. First, it was verified if ergosterol was efficient in these cells inducing modifications of proton fluxes and increased expression of defence-related genes. Then, it was shown that ergosterol induced a rapid and transient biphasic increase of free [Ca2þ]cyt which intensity dep…

0106 biological sciencesTime FactorsPhysiologyNicotiana tabacumPlant SciencesterolsSecond Messenger Systemstobacco01 natural scienceschemistry.chemical_compoundCytosolpolycyclic compoundsPhosphorylationCalcium signalingreactive oxygen species0303 health sciencesErgosterolelicitorbiologyergosterolHydrogen-Ion ConcentrationPlants Genetically ModifiedRecombinant ProteinsCell biologyBiochemistrySecond messenger systemReactive oxygen species; Calcium signature; Elicitor; Signal transduction; MAPKs; tobaccolipids (amino acids peptides and proteins)Protonssignal transductionCell Survivalnicotiana plumbaginifoliachemistry.chemical_elementnicotiana tabacumoxydantCalciumcalcium signature03 medical and health sciencesAequorinMAPKsBAPTAGenetics[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyCalcium Signaling030304 developmental biologyMitogen-Activated Protein Kinase KinasesCalcium metabolismHydrogen Peroxidebiochemical phenomena metabolism and nutritionbiology.organism_classificationCytosolchemistryCalciumApoproteins010606 plant biology & botany
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Molecular characterization of <em>Vitis vinifera</em> L. local cultivars from volcanic areas (Canary Islands and Madeira) using SSR marke…

2019

Aim: This study characterises and identifies 79 grapevine accessions from the Canary Islands and 14 from Madeira using simple sequence repeat (SSR) analysis.Methods and Results: A kit of 20 microsatellites or simple sequence repeats (SSRs) were used to obtain the molecular profiles of the 93 accessions in this study. The results allowed us to identify four new cultivars (Bienmesabe tinto, Burra volcanica, Vallera, Verijadiego negro), two new colour mutations (Listan rosa, Mollar cano rosado) and two unknown molecular profiles from Madeira. Furthermore, we propose that eight names of varieties be included in the Vitis International Variety Catalogue (VIVC) as prime names, and 38 accession na…

0106 biological sciencesVinebiologyVitis International Variety CatalogueBiodiversity04 agricultural and veterinary sciencesHorticulturebiology.organism_classification01 natural sciencesHorticultureGenetic structure040103 agronomy & agriculture0401 agriculture forestry and fisheriesCriollo tobaccoMicrosatelliteCultivarPhylloxera010606 plant biology & botanyFood ScienceOENO One
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Environmental Tobacco Smoke During the Early Postnatal Period of Mice Interferes With Brain 18 F-FDG Uptake From Infancy to Early Adulthood – A Longi…

2020

Exposure to environmental tobacco smoke (ETS) is associated with high morbidity and mortality, mainly in childhood. Our aim was to evaluate the effects of postnatal ETS exposure in the brain 2-deoxy-2-[18F]-fluoro-D-glucose (18F-FDG) uptake of mice by positron emission tomography (PET) neuroimaging in a longitudinal study. C57BL/6J mice were exposed to ETS that was generated from 3R4F cigarettes from postnatal day 3 (P3) to P14. PET analyses were performed in male and female mice during infancy (P15), adolescence (P35), and adulthood (P65). We observed that ETS exposure decreased 18F-FDG uptake in the whole brain, both left and right hemispheres, and frontal cortex in both male and female i…

0301 basic medicineCingulate cortexmedicine.medical_specialtyCerebellumpositron emission tomographyglucose metabolismbrainThalamusHippocampusenvironmental tobacco smokeStriatum18F-FDG uptakelcsh:RC321-571Midbrain03 medical and health sciences0302 clinical medicineInternal medicineCortex (anatomy)medicinelcsh:Neurosciences. Biological psychiatry. NeuropsychiatryOriginal ResearchBasal forebrainpassive smokeneuroimagingbusiness.industryGeneral Neurosciencelongitudinal studyCÉREBRO030104 developmental biologyEndocrinologymedicine.anatomical_structurebusiness030217 neurology & neurosurgeryNeuroscienceFrontiers in Neuroscience
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Is lithium implicated in tobacco addiction?

2016

IntroductionTobacco is a source of mineral elements that can affect human health in various ways, such as lithium, which is used as a psychiatric medication. Lithium salts are used as mood-stabilizing drugs and indicated in the treatment of manic-depressive psychosis.ObjectiveStudying the lithium content in tobacco over the smokers’plasma content and evaluate the potential role of lithium in tobacco addiction.MethodsA total of 18 different tobacco products (cigarettes, smokeless and water pipe tobacco) and 125 plasma samples (45 from smokers, 10 from ex-smokers and 70 from non-smokers) were collected to determinate the lithium content. Tobacco samples were digested with nitric acid and lith…

Addictionmedia_common.quotation_subjectSignificant differencechemistry.chemical_elementSignificant negative correlationLithium levelPsychiatry and Mental healthchemistrySmokeless tobaccoWater pipeLithium Tokamak ExperimentLithiumFood sciencemedia_commonEuropean Psychiatry
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Sister chromatid exchange, (SCE), High-Frequency Cells (HFCs) and SCE distribution patterns in peripheral blood lymphocytes of Spanish adult smokers …

2014

According to the International Agency for Research on Cancer, smoking tobacco is a major cause of cancer in humans. It causes about half of all male cancer deaths and an ever increasing number of cancer deaths in females. The aim of this study was to establish whether cigarette smoking increases sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in two Spanish population groups; light and heavy smokers. The mean number of High-Frequency Cells (HFCs) was determined and, the SCE distribution pattern among the chromosomes was analysed represented by a ratio described below. A local sample of 101 adult smokers (n = 48) and non-smokers (n = 53), aged from 18 to 49 years, was studi…

AdultMaleAdolescent[SDV]Life Sciences [q-bio]PhysiologySister chromatid exchangeBiologyToxicologyINGENIERIA NUCLEARToxicology03 medical and health sciencesYoung Adult0302 clinical medicineTobaccomedicineDistribution (pharmacology)Sister chromatidsHumansLymphocytes030304 developmental biologySCEs0303 health sciencesHeavy smokingSmoking TobaccoSmokingCytogenetic assessmentCancerGeneral MedicineBiomarkerMiddle Agedmedicine.diseasePeripheral blood3. Good healthSpanish populationSpain030220 oncology & carcinogenesisCase-Control StudiesFemaleSister Chromatid ExchangeFood Science
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Population attributable risk of tobacco and alcohol for upper aerodigestive tract cancer.

2011

Tobacco and alcohol are major risk factors for upper aerodigestive tract (UADT) cancer and significant variation is observed in UADT cancer rates across Europe. We have estimated the proportion of UADT cancer burden explained by tobacco and alcohol and how this varies with the incidence rates across Europe, cancer sub-site, gender and age. This should help estimate the minimum residual burden of other risk factors to UADT cancer, including human papillomavirus. We analysed 1981 UADT cancer cases and 1993 controls from the ARCAGE multicentre study. We estimated the population attributable risk (PAR) of tobacco alone, alcohol alone and their joint effect. Tobacco and alcohol together explaine…

AdultMaleCancer Researchmedicine.medical_specialtyAlcohol DrinkingEsophageal NeoplasmsAlcoholchemistry.chemical_compoundRisk FactorsTobaccomedicineHumansPopulation attributable riskGynecologyMouth neoplasmbusiness.industryIncidenceMedicine (all)Incidence (epidemiology)SmokingCase-control studyCancerAlcohol; ARCAGE study; Population attributable risk; Tobacco; Upper aerodigestive tract cancer; Adult; Alcohol Drinking; Case-Control Studies; Esophageal Neoplasms; Europe; Female; Humans; Incidence; Male; Middle Aged; Mouth Neoplasms; Otorhinolaryngologic Neoplasms; Risk Factors; Smoking; Oral Surgery; Medicine (all); Oncology; Cancer ResearchMiddle Agedmedicine.diseaseUpper aerodigestive tract cancerARCAGE studyEuropeOtorhinolaryngologic NeoplasmsUpper aerodigestive tractOncologychemistryCase-Control StudiesAttributable riskFemaleMouth NeoplasmsUpper aerodigestive tract cancer; ARCAGE study; Population attributable risk; Tobacco; AlcoholOral SurgeryAlcoholbusinessOropharyngeal CancersDemography
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Tobacco smoking and alcohol consumption as risk factors for thymoma - A European case-control study

2019

PURPOSE: Hardly anything is known about the aetiology of thymoma. This paper presents data regarding tobacco smoking and alcohol consumption in relation to thymoma from the first case-control study performed on this rare tumour.METHODS: A European multi-centre case-control study including incident cases aged 35-69 years with thymoma between 1995 and 1997, was conducted in seven countries. A set of controls, used in seven parallel case-control studies by the same research group was used, including population-based controls from five countries and hospital controls with colon cancer from two countries. Altogether 103 cases, accepted by a reference pathologist, 712 colon cancer controls, and 2…

AdultMaleCancer Researchmedicine.medical_specialtyThymomaAlcohol DrinkingThymomaEpidemiologyColorectal cancerThymus cancerPopulationMedizin03 medical and health sciences0302 clinical medicineInternal medicinemedicineHumans030212 general & internal medicineeducationAgedAlcohol; Case-control study; Risk factors; Thymoma; Thymus cancer; Tobacco smokingeducation.field_of_studybusiness.industryCase-control studyCase-control studyMiddle Agedmedicine.diseaseTobacco smokingIncreased riskOncologyRisk factorsCase-Control Studies030220 oncology & carcinogenesisEtiologyFemalebusinessAlcoholAlcohol consumption
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The impact of pictorial health warnings on tobacco products in smokers behaviours and knowledge: The first quasi-experimental field trial after the i…

2019

Background. The aim of the study was to evaluate in the Italian smokers, the effects of implementation of the law about Pictorial Health Warnings (PHWs) on tobacco products.Methods. A quasi-experimental longitudinal design was conducted between 2016 and 2017. The data were collected before (pre-PHW/Wave 1) and after (post-PHW/Wave 2) the implementation of the law. The adopted questionnaire included impact of advertisement (Label Impact Index, LII), quitting behavior and knowledge of tobacco related diseases.Results. 455 respondents completed both the Waves. 7.7% of smokers declared to have stopped smoking in Wave 2 and 29% of these declared the PHWs as one of the reasons to quit. The knowle…

AdultMaleHealth Knowledge Attitudes Practicehealth warningQuasi-experimental studyProduct LabelingPictorial warningsPictorial Works as TopicYoung AdultPictorial warningAdvertisingSurveys and QuestionnairesHealth labelHumansLongitudinal StudiesHealth labelsHealth warningsPracticeMotivationpictorial warnings health labels health warnings tobacco advertisement quasi-experimental studyHealth KnowledgeTobacco advertisementTobacco ProductsTobacco Use DisorderMiddle AgedItalyAttitudesFemaleSmoking CessationHealth labels; Health warnings; Pictorial warnings; Quasi-experimental study; Tobacco advertisementpictorial warnings; health labels; health warnings; tobacco advertisement; quasi-experimental studyHealth labels; Health warnings; Pictorial warnings; Quasi-experimental study; Tobacco advertisement; Adult; Advertising; Female; Health Knowledge Attitudes Practice; Humans; Italy; Longitudinal Studies; Male; Middle Aged; Motivation; Pictorial Works as Topic; Smoking Cessation; Surveys and Questionnaires; Tobacco Products; Tobacco Use Disorder; Young Adult; Product Labeling
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18-yr cumulative incidence of respiratory/allergic symptoms/diseases and risk factors in the Pisa epidemiological study

2019

Abstract Background Few population-based studies on the effects of environmental exposure variation exist. Aim Assessing respiratory symptom/disease incidence related to risk factor exposure changes. Methods A longitudinal general population sample from two surveys (PISA2:1991–1993; PISA3:2009–2011; no. = 970), aged ≥20 years at baseline, completed a questionnaire on respiratory symptoms/diseases, risk factor exposure and performed spirometry. 18-year follow-up cumulative incidence of respiratory symptoms/diseases and longitudinal changes (persistence, incidence, remittance) in risk factor exposure were computed. Results Cumulative incidence values were: 3.2% (corresponding to a 1.8‰/year i…

AdultMalePulmonary and Respiratory MedicineSpirometrymedicine.medical_specialtyTime FactorsAllergyPopulationYoung Adult03 medical and health sciences0302 clinical medicineRisk FactorsSurveys and QuestionnairesInternal medicineWheezeTobaccomedicineCOPDHumansCumulative incidence030212 general & internal medicineeducationEnvironmental and occupational Health and EpidemiologyAgedAsthmaCOPDeducation.field_of_studymedicine.diagnostic_testbusiness.industryIncidence (epidemiology)SmokingEnvironmental ExposureEnvironmental exposureMiddle AgedRespiration Disordersmedicine.diseaseAsthmarespiratory tract diseases030228 respiratory systemAllergy; Asthma; COPD; Environmental & occupational health and epidemiology; TobaccoFemalemedicine.symptombusinessEnvironmental & occupational health and epidemiologyRespiratory Medicine
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